CHARACTERIZATION AND MOLECULAR CLONING OF THERMOSTABLE ALPHA-AMYLASE FROM STREPTOMYCES SP TO1

Mellouli L.; Kammoun A.; Mezghani M.; Bejar S.; Ghorbel R.

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CHARACTERIZATION AND MOLECULAR CLONING OF THERMOSTABLE ALPHA-AMYLASE FROM STREPTOMYCES SP TO1

机译：从链霉菌SP到1的常规α-淀粉酶的表征与分子克隆

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A new thermophilic Streptomyces sp. T01, isolated from Tunisian soil, produced a thermostable alpha-amylase and pullulanase. The gene encoding for the alpha-amylase activity was cloned into the multicopy cloning plasmid pLM1 using S. lividans ZX1 as host strain. The ZX1 / pLM1 strain has the same activity than the initial T01 strain and about 25 fold higher activity than the ZX1 strain. This alpha-amylase has an optimum of pH and temperature at 6 and 70 degrees C respectively. [References: 21]

机译：一种新的嗜热链霉菌sp。从突尼斯土壤中分离的T01产生了恒温α-淀粉酶和胶酶。使用S. Lividans ZX1作为宿主菌株，将用于α-淀粉酶活性的基因克隆到多拷贝克隆质粒PLM1中。 ZX1 / PLM1应变具有比初始T01应变相同的活性，并且比ZX1菌株高约25倍。该α-淀粉酶分别在6和70℃下具有pH和温度的最佳值。 [参考：21]

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《Biotechnology Letters》 |1996年第7期|共6页
作者
Mellouli L.; Kammoun A.; Mezghani M.; Bejar S.; Ghorbel R.;
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CTR BIOTECHNOL SFAX BP W SFAX 3038 TUNISIA;

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原文格式 PDF
正文语种 eng
中图分类生物工程学（生物技术）;生命科学总论;分子生物学;
关键词
Bacillus-subtilis; Gene; Pullulanase; Enzyme;

机译：枯草芽孢杆菌;基因;淀粉酶;酶;

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1. CHARACTERIZATION AND MOLECULAR CLONING OF THERMOSTABLE ALPHA-AMYLASE FROM STREPTOMYCES SP TO1 [J] . Mellouli L., Kammoun A., Mezghani M., Biotechnology Letters . 1996,第7期

机译：从链霉菌SP到1的常规α-淀粉酶的表征与分子克隆
2. Isolation,Characterization,and Molecular Cloning of a Thermostable Xylitol Oxidase from Streptomyces sp. IKD472 [J] . YAMASHITA MITSUO, OMURA HIRONORI Journal of Bioscience and Bioengineering . 2000,第4期

机译：链霉菌热稳定木糖醇氧化酶的分离，鉴定及分子克隆IKD472
3. Alpha-amylase gene of thermophilic Streptomyces sp TO1: nucleotide sequence, transcriptional and amino acid sequence analysis [J] . Mellouli L., Virolle MJ., Bejar S., FEMS Microbiology Letters . 1998,第1期

机译：嗜热链霉菌TO1的α-淀粉酶基因：核苷酸序列，转录及氨基酸序列分析
4. Cloning, expression and characterization of a thermostable pullulanase from newly isolated thermophilic Geobacillus sp. LM14–3 [C] . Sun Shaojing, Lu Fuping, Song Hui, 2011 4th International Conference on Biomedical Engineering and Informatics . 2011

机译：从新分离的嗜热芽孢杆菌属的嗜热支链淀粉酶的克隆，表达和鉴定。 LM14–3
5. Factors effecting thermostability of a family 1 beta-glycosidase cloned from a Fervidobacterium sp. isolated from Yellowstone National Park. [D] . Sullivan, Raymond Francis. 2003

机译：影响从Fervidobacterium sp。克隆的家族1β-糖苷酶热稳定性的因素。从黄石国家公园隔离。
6. Raw-starch-digesting and thermostable alpha-amylase from the yeast Cryptococcus sp. S-2: purification characterization cloning and sequencing. [O] . H Iefuji, M Chino, M Kato, 1996

机译：来自酵母隐球菌sp。的生淀粉消化和热稳定的α-淀粉酶。 S-2：纯化鉴定克隆和测序。
7. Alpha-amylase gene of thermophilic Streptomyces sp. TO1: nucleotide sequence, transcriptional and amino acid sequence analysis [O] . L Mellouli 1998

机译：嗜热链霉菌SP的α-淀粉酶基因。 TO 1：核苷酸序列，转录和氨基酸序列分析
8. Gene Technological Studies of an alpha-Amylase Based Expression System in'Streptomyces'. 2. Cloning and Regulation of an alpha-Amylase Gene in 'Streptomyces lividans'. (Gentekniska Studier av ett alpha-Amylasbaserat Proteinproduktionssystem i 'Streptomyce [R] . Osterman, A., Granstroem, M., Forsman, M. 1992

机译：“链霉菌”中基于α-淀粉酶的表达系统的基因技术研究。 2.“浅青紫链霉菌”中α-淀粉酶基因的克隆和调控。（Gentekniska studier av ett alpha-amylasbaserat proteinproduktionssystem i'streptomyce

CHARACTERIZATION AND MOLECULAR CLONING OF THERMOSTABLE ALPHA-AMYLASE FROM STREPTOMYCES SP TO1

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