Sequence and Structure Analysis of CRP of Lung and Breast Cancer Using Bioinformatics Tools and Techniques

摘要

In the present study, bioinformatics approach has been adopted to explore the sequences and structures analysis of CRP of lung and breast cancer and compares with normal sequence from NCBI. The present study was aimed to investigate the possibility ofusing CRP as a marker for patients with lung and breast cancer. Also the effect of mutation on the physicochemical properties and structure of CRP. 40 blood and serum samples were examined from patients with lung and breast cancer (aged between 23and 45years old). Qualitative test was done to detect the presence of CRP in the patient’s serum. The qualitative test showed that 3(7.5%) patients give positive result and 37(92.5%) patients with lung and breast cancer give negative result to presence of CRP in the serum.In patient with lung cancer five missense mutations and four deletion mutations detected. While in the patient with lung cancer four missense mutations ,six deletion mutations and eight insertion mutation detected by BLAST. One point mutation appeared in all patients at same site and has same effect, this meaning there are relationship between this mutation and cancer disease. This mutation recorded in NCBI, DDBJ and ENA with the numbers LC276938 and LC276937. The present study determinedthe physico-chemical properties of CRP such as their hydrophilic nature; alpha-helical structure and 3D structure. The results of present study Show that CRP consider non-specific marker for patient with Lung and breast cancenAlso the mutations on CRP gene effected on the structure and physico-chemical properties of C-reactive protein.