Providing Lipids Boosts Protein ProductivityTesting a Feed Supplement with Multiple Cell Clones and Media Formulations

摘要

As the biologies (and now biosimilar) markets continue to grow, pressure increases on biomanufacturers to reduce cost of goods sold (CoGS). One way they can reduce cost is by increasing protein productivity in terms of protein titer per volume of culture. Media optimization is a key strategy for increasing protein productivity. In the past few decades, average titers across the industry have increased greatly— from <0.5 g/L in the 1980s to >3 g/L today, and it is not uncommon now to see titers of ~5g/L (l). Despite those improvements in protein output, the drive to continue optimizing processes remains, not only to further increase expression titers, but also to improve protein quality.