Optimal Feed Strategies to Enhance Protein Production of CHO Clones Using Thermo Scientific HyCell CHO and Cell Boost Feeds

摘要

Process development and optimization is critical to enhancing the productivity of various cell lines. Nutrient requirements for peak productivity can be clone and process specific. Implementing the right feed formulation,and strategy can greatly enhance the productivity of cell cultures in a fed-batch process. A small-scale Design of Experiment (DoE) method was used to determine the optimal feed strategy (formulation, concentration, and timing) to maximize both product titer and peak viable cell density for several CHO clones. To alleviate concerns about media component variability, only chemically defined HyCell CHO medium and Hy-Clone Cell Boost feeds were screened. Initial screenings were conducted at small-scale (1 mL cultures), allowing for a higher number of treatments and replicates. Once the optimal process for a particular clone was determined, scale-up to shaker flasks and bench-top bioreactors was performed, in order to compare batch and fed-batch product expression levels, and scalability of the process.