Toward a Roadmap for Cell-Free Synthesis in Bioprocessing

摘要

Cell-tree syntnesis (Ltb), also known as cell-free transcription and translation, supplements cellular components (either a cell lysate or purified recombinant elements) with nucleotides, amino acids, metabolic intermediates, and salts to produce a nucleic acid or protein from a genetic template added to the reaction. This exciting technology has seen a substantial increase in both academic and commercial interest over the past decade (1). Interest stems in large part from the potential to democratize access to the machinery of biology by removing the need to engineer cells genetically (2). CFS has the potential to revolutionize healthcare in much the same way that personal computers did for information technology (3). However, our interest and thatof the bioprocessing community at large comes from the potential to transform manufacturing for some healthcare products (4).