Beneficial effects of fermented camel milk by lactococcus lactis subsp cremoris on cardiotoxicity induced by carbon tetrachloride in mice

摘要

Carbon tetrachloride (CCl4) is a xenobiotic present in the environment, can cause harmful effects on human health. In the present study, we attempted to elucidate the cardiopreventive potential of the fermented camel milk by lactococcus lactis subsp cremoris (FCM-LLC) against the toxic effects of acute exposure to CCl4 on heart tissue of mice. Twenty-eight mice's were divided into four groups of seven each: group (C) served as control; group (FCM-LLC) received only 100 mg L of FCM-LLC/kg body weight daily for 15 days; group (CCl4) was administered by a single dose of CCl4 (10 mL/kg in 0.3% olive oil, i.p) at day 14 and group (FCM-LLC + CCl4) pretreated with FCM-LLC and received a single dose of CCl4 on day 14. The exposure to a single dose of CCl4 caused cardiotoxicity expressed by an increase in lipid peroxidation (TBARS), protein carbonyls (PC) levels and in antioxidant markers (superoxide dismutase (SOD), catalase (CAT), gluthathione peroxidase (GPx), glutathione (GSH) and Vitamin C levels) in the CCl4-treated group when compared with the untreated group. Furthermore, treatment with CCl4 significantly elevated the cardiac toxicity markers while increasing of alanine aminotransferase (ALT), lactate dehydrogenase (LDH), creatine kinase (CK), creatine kinase MB (CKMB) and Troponin I activities. The pre-treatment of experimental mice's with FCM-LLC has allowed an improvement through lowering oxidative stress and attenuating cardiac toxicity. These modifications were further evident through histopathological aspects of the heart. Overall, the present data provide evidence of the beneficial effects of fermented camel milk by lactococcus lactis subsp creemoris clearly revealed through the reduction of the CCl4 induced heart oxidative damages.