An oxygen-releasing device to improve the survival of mesenchymalstem cells in tissue engineering

摘要

Supplying oxygen to inner areas of cell constructs to support cell proliferation and metabolism is amajor challenge in tissue engineering involving stem cells. Developing devices that incorporate oxygenrelease materials to increase the availability of the localized oxygen supply is therefore key toaddressing this limitation. Herein, we designed and developed a 3D-printed oxygen-releasing devicecomposed of an alginate hydrogel scaffold combined with an oxygen-generating biomaterial(calciumperoxide) to improve the oxygen supply of the microenvironment for culturing adipose tissue-derivedstem cells. The results demonstrated that the 3D-printed oxygen-releasing device alleviated hypoxia,maintained oxygen availability, and ensured proliferation of the embedded cells, whilst also reducinghypoxia-induced apoptosis. The introduction of this 3D-printed oxygen-releasing device couldenhance the survival of embedded stem cells.