Electrochemical biosensing utilizing synergic action of carbon nanotubes and platinum nanowires prepared by template synthesis

摘要

Platinum nanowires (PtNWs) prepared by electrodeposition method with the help of porous anodic aluminum oxide (AAO) templates have been solubilized in chitosan (CHIT) together with carbon nantubes (CNTs) to form a PtNW-CNT-CHIT organic-inorganic system. The resulting PtNW-CNT-CHIT material brings capabilities for utilizing synergic action of PtNWs and CNTs to facilitate electron-transfer process in electrochemical sensor design. The PtNW-CNT-CHIT film modified electrode offered a significant decrease in the overvoltage for the hydrogen peroxide and showed to be excellent amperometric sensors for hydrogen peroxide at-0. 1 V over a wide range of concentrations, and the sensitivity is 260 mu A mM(-1) cm(-1). As an application example, by linking glucose oxidase (GOx), an amplified biosensor toward glucose was prepared. The glucose biosensor exhibits a selective determination of glucose at -0. 1 V with a linear response range of 5 x 10(-6) to 1.5 x 10(-2) M with a correlation coefficient of 0.997, and response time < 10 s. The high sensitivity of the glucose biosensor is up to 30 mu A mM(-1) cm(-2) and the detection limit was 3 mu M. The biosensor displays rapid response and expanded linear response range, and excellent repeatability and stability. (c) 2006 Elsevier B.V. All rights reserved.