Evolutionary graphs (EGs), in which evolutionary dynamic is arranged on a graph, were initially proposed by Lieberman et al. [Lieberman, E., Hauert, C., Nowak, M.A., 2005. Evolutionary dynamics on graphs. Nature 433, 312–316] in the biological field and many biological phenomena are successfully explained. EGs on two levels (or bi-level EGs), based on some biological phenomena, are considered in this paper. The bi-level EGs are compared with the one-rooted EGs in two cases. One has the identical numbers of the followers, the other with the same numbers of total individuals. Then, some properties of the bi-level EGs are obtained. It is showed that bi-level EGs are more stable, and the bi-level EGs with just two leaders are the most stable, if they have identical followers respectively. The bi-level EGs theory can successfully explain the phenomena of symbiosis in biology.
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