Isolation of the psychrotolerant species Bacillus weihenstephanensis from raw cow's milk

摘要

Fifty-six mesophilic and psychrotolerant Bacillus cereus strains were isolated during a monitoring of Bacillus spp. in bulk milk samples from Moravian and Bohemian farms, Czech Republic. Thirty-four mesophilic strains grew at 40-43degreesC and did not grow at 4-7degreesC. Twenty of the 22 psychrotolerant strains were able to grow between 10 and 40degreesC. They did not grow at 4 and 43degreesC. Only two cold-tolerant strains that were classified as Bacillus weihenstephanensis were able to grow at 4-7degreesC. Two PCR assays (cspA-PCR and 16S rDNA-PCR) and ribotyping confirmed their correct identification as B. weihenstephanensis. These strains are deposited in the Czech Collection of Microorganisms (CCM) as B. weihenstephanensis CCM 4965 and CCM 4966.