A nanosensor based on quantum-dot haptens for rapid, on-site immunoassay of cyanotoxin in environmental water

摘要

A nanoprobe based on quantum-dot (QD) haptens was synthesized by conjugating carboxyl quantum dots with aminoethyl-microcystin (MC)-leucine-arginine (LR). A two-alkyi group was introduced to supply a spacer between the QD nanoprobe and anti-MC-LR antibody to reduce the steric hindrances of immunoreaction. The sensor system based on a portable optofluidic platform exhibited a liner range of 0.10-4.0 礸/L for MC-LR with a detection limit of 0.03 礸/L. The proposed sensor has potential application in the rapid, on-site detection of MC-LR in real water samples.