H-1 nuclear magnetic resonance-based plasma metabolomics provides another perspective of response mechanisms of newborn calves upon the first colostrum feeding

摘要

The first meal of a neonatal calf after birth is crucial for survival and health. Blood IgG levels remarkably increase in neonatal calves after the first colostrum feeding. However, there is little comprehensive information on blood small-molecule metabolites in neonatal calves at that time. In this study, the changes in plasma metabolites of neonatal calves after the initial colostrum feeding were first examined with comprehensive H-1 nuclear magnetic resonance (NMR). Sixteen plasma samples obtained from 8 calves before and after feeding were analyzed with H-1 NMR. Multivariate analyses revealed a significant difference in metabolic profiles. After feeding, acute phase N-acetylated glycoproteins 13 other plasma metabolites decreased, whereas 19 plasma metabolites increased. Metabolomics pathway analysis of these metabolites revealed that a global metabolic response on the first colostrum feeding was reflected by alterations of 13 metabolic pathways including lipid, carbohydrate, and amino acid metabolism in neonatal calves. These results suggested that besides meeting energy demand, a 4.0 L of high-quality colostrum feeding within 4 h after birth had a positive effect on relieving the postnatal stress in neonatal calves. This study provides another perspective of response mechanisms of newborn calves upon the first colostrum feeding.