Polyelectrolyte multilayer coatings for short/long-term release of antibacterial agents

摘要

Osteomyelitis caused by orthopedic and cranial implants infection is the principal cause of implant failure, septicemia, and even death; it is an urgent issue with prevalence rates ranging from 4% to 10% in industrialized countries. Bioactive and multifunctional materials such as bioglass, biometal, and biopolymer are commonly used in biomedical fields; however, it is still a challenge to fabricate effective antibacterial coatings. The purpose of this study is to use layer-by-layer technology to develop an antibacterial coating with outer and inner portions for short/long-term release of antibacterial agents. Antibiotics would be released immediately after implant and antibacterial silver ions in the long term. In this study, polyelectrolyte multilayers (PEMs) comprised of collagen as the cationic layers and gamma-poly-glutamic acid as the anionic layers were coated onto the 316L stainless steel substrate via a spin coating technique. A chitosan buffer layer was designed and placed in the middle of the coating. 1 mol% silver additive 58S bioactive glass (SiO2: CaO: P2O5 = 60: 36: 4 in mol%) was introduced into the inner gamma-poly-glutamic acid layers, while gentamicin was added to the outer layer.