Microbial genetic resource mapping of East Calcutta wetlands.

摘要

East Calcutta Wetland (ECW) is the world's largest natural treatment plant for solid and soluble waste, where bioremediation and biodegradation of complex compounds is mainly based on microbial activity and is recognized as a potential source of bacteria of biotechnological impact. Here we discuss the microbial resource mapping of this important Ramsar site. Culture-independent analysis of the microbial population from soil and water through community DNA isolation, amplification, cloning and partial sequencing (320 clones) of 16Sr RNA gene sequence and finally secondary structure-based phylogenetic analysis of the novel sequences (292 GenBank submissions) using ARB software indicated the presence of microorganisms from 12 different main bacterial phyla, thus revealing the rich natural microbial resource at ECW..