Detection of microRNA using enzyme-assisted amplifying and DNA-templated silver nanoclusters signal-off fluorescence bioassay

摘要

A Simple and fast analysis strategy of fluorescence quenching based on DNA-templated silver nanoclusters was developed for detection of miR-122 related to diseases such as human liver. We used Exo III to cleave the silver cluster template and assist in the DNA-RNA complex cycle. When the target is absent, the silver cluster template remains intact, and DNA-AgNCs are generated under the action of AgNO3/NaBH4, producing a strong background fluorescence signal. Once the target is added, the site of the Exo III occurs after a series of hybridization cycles, the Exo III acts, the template DNA is continuously hydrolyzed, and the fluorescence intensity of the system is significantly reduced. By comparing the changes in the fluorescence signal, we found that this strategy has good sensitivity and the detection limit is as low as 84.0 pM. The strategy also has excellent discriminating ability and good selectivity, it can provide a persuasive reference for the early diagnosis of liver cancer and hepatitis.