Tilt-invariant scanned oblique plane illumination microscopy for large-scale volumetric imaging

摘要

This Letter presents the first demonstration of multi-tile stitching for large scale 3D imaging in single objective light sheet microscopy. We show undistorted 3D imaging spanning complete zebrafish larvae and over 1 mm(3) volumes for thick mouse brain sections. We use remote galvo scanning for light-sheet creation and develop a processing pipeline for 3D tiling across different axes. With the improved one photon (1p) tilt-invariant scanned oblique plane illumination (SOPi, /sopi) microscope presented here, we demonstrate cellular resolution imaging at depths exceeding 330 mu m in optically scattering mouse brain samples and dendritic imaging in more superficial layers. (C) 2019 Optical Society of America