INTERACTION OF GABA AND NOREPINEPHRINE IN THE LATERAL DIVISION OF THE BED NUCLEUS OF THE STRIA TERMINALS IN ANESTHETIZED RAT, CORRELATING SINGLE-UNIT AND CARDIOVASCULAR RESPONSES

摘要

The bed nucleus of the stria terminalis (BST) consists of multiple anatomically distinct nuclei. The lateral division, which receives dense noradrenergic innervation, has been implicated in cardiovascular regulation and modulation of responses to stress. This study is performed to identify the cardiovascular and single-unit responses of the lateral BST to norepinephrine (NE), involved adrenoceptors, and possible interaction with GABAergic system of the BST in urethane-anesthetized rats. NE, adrenoreceptor antagonists, and GABA(A) antagonist were microinjected into the lateral division of BST, while arterial pressure (AP), heart rate (HR), and single-unit responses were simultaneously recorded. NE microinjected into the lateral division of BST produced depressor and bradycardic responses. The decrease in AP and HR to NE was blocked by prazosin, an alpha(1)-adrenoreceptor antagonist, but not by yohimbine, an alpha(2) antagonist. Furthermore, injections of the GABA(A) receptor antagonist, bicuculline methiodide (BMI), into the lateral BST abolished the NE-induced depressor and bradycardic responses. We also observed single-unit responses consisting of excitatory and inhibitory responses correlated with cardiovascular function to the microinjection of NE. In conclusion, these data provide the first evidence that microinjection of NE in the lateral division of BST produces depressor and bradycardic responses in urethane-anesthetized rat. The depressor and bradycardiac response are mediated by local alpha(1)- but not alpha(2)-adrenoceptors. alpha(1)-AR activates the GABAergic system within the BST, which in turn produces depressor and bradycardic responses. (C) 2017 IBRO. Published by Elsevier Ltd. All rights reserved.