Effects of phosphatidic acid on recombinant protein production by chinese hamster ovary cells in serum-free culture

摘要

When recombinant Chinese hamster ovary (CHO) cells (ATCC CRL-8200) producing human interferon-#gamma# (hIFN-#gamma#) were incubated with exogenously supplied phosphatidic acid (PA) from egg yolk lecithin in a serum-free medium, PA induced increases in both the density of viable cells and concentration of hIFN-#ganna# secreted into the medium. Dispersion of PA with a non-ionic surfactant, Tween 80, further enhanced both cell growth and hIFN-#gamma# production. Replacement of the culture medium containing PA by fresh medium without PA in the course of a static culture did not influence cell growth indicating that PA is required to be continuously present in a serum-free medium to stimulate cell growth. Using a fresh medium containing PA for replacement resulted in significant enhancement of both cell density and hIFN-#gamma# yield. These results suggest that PA is a promising constituent of low-protein serum-free media for the effective production of recombinant proteins.