Production of antihypertensive and antioxidant activities by enzymatic hydrolysis of protein concentrates recovered by ultrafiltration from cuttlefish processing wastewaters

摘要

This work focuses on the production of antihypertensive and antioxidant activities using enzymatic hydrolysis of protein concentrates recovered by ultrafiltration of different wastewaters from the industrial processing of cuttlefish (1llex argentinus). The effluents were produced in the processes of thawing (E1), softening (E2), boiling (E3) and gelation (E4). Our results showed that membranes with cut-off at 100,30 and 10 kDa were an effective resource to protein concentration of E2 and E3 but limited for El and E4. In addition, E2 and E3 retentates led to remarkable antihypertensive and antioxidant activities, further improved by enzymatic hydrolysis. Also sequential ultrafiltration revealed the enrichment of these protein concentrates in peptides with high angiotensin-converting enzyme (ACE)-inhibitory activity. Thereby, UF-fractionation followed by proteolysis of protein concentrates from cuttlefish wastewaters offers new opportunities for the development of bioactive hydrolysates with application in the food industry. In addition, this approach contributes to an improved depuration of industrial wastewaters, reducing the treatment costs and leading to a decrease in its contaminating effect.