Kinetics of hydrolysis of tetrahydrofurfuryl butyrate in a three phase system containing immobilized lipase from Candida antarctica

摘要

Hydrolysis of tetrahydrofurfuryl butyrate (THFB) in heptane was carried out a 30 deg C in a triphasic system by using stoichiometric amounts of water and immobilized Candida antarctica lipase (Novozym 435) as catalyst.The phase system has several advantages in hdyrolysis reaction due to the very high solubility of the hdyrophobic substrate in the organic medium and suppression of enzyme inhibition by the products alcohol and acid,which are partitioned into the ublk organic phase.The effects of various parameters such as speed of agitation,cocnentration of the ester,quantity of water,temperature,catalyst loading,and reusability of the enzyme were systematically studied in detail.The catalyst reysability studies showed that the deactivation was reversible.The reaction free from external mass transfer and intra-particle diffusionh limitations.Based on the initial rates and cocnentration prfiles,the hydrolysis reaction was found to obey the ping-pong bi-bi mechanism with inhibition by the reactant water.This also demonstrates the significance of the three phase hydrolysis by enzyme catalysis.It indicates that by taking stoichiometric amounts of reagnets,inhibition due to reactants can be reduced in a three phase system and the reusability of the catalyst also can be improved.