in silico Single Nucleotide Polymorphism Prediction and Design for Targeting Amyloid Precursor Protein in Alzheimers Disease

摘要

Alzheimer's disease is a progressive neurodegenerative disorder, is the common cause of dementia and affect life quality. Present research analysis is based on the amyloid precursor protein as it is one of the major biomarkers of Alzheimer's disease, the insoluble part of which gets deposited due to the inappropriate cleavage by secretase enzyme. The instrumental part of this study was performed by using Computer Aided Drug Design (CADD). About 23 phytoconstituents were taken, from these constituents rosamarinic acid produced the highest docking score. Preliminary characterizations of all the ligands were conducted using Biovia Discovery studio along with protein characterization, Lipinski rule analysis and ADMET. The in silico analysis convincingly predicted the action of rosamarinic acid, against the formation of amyloid plaque by binding with the amyloid precursor protein .Details of single nucleotide polymorphism on gene APPI were analyzed by dbSNP database and displays the need for further in vitro study.