Effect of varying flow regimes upon elution behaviour, apparent molecular characteristics and hydrodynamic properties of amylopectin isolated from normal corn starch using asymmetrical flow field-flow fractionation

摘要

A detailed study of the elution behaviour, apparent molecular characteristics and hydrodynamic properties of amylopectin-type fraction (isolated from normal corn starch) in aqueous media employing asymmetrical flow field-flow fractionation (AF4) was undertaken by systematically varying the channel flow (F_(ch)), cross flow (F_(cr)) and F_(cr)/F_(ch) ratios. Distributions of apparent molar masses and radii of gyration, mass recoveries and hydrodynamic radii decreased as a function of increasing F_(cr) at a fixed F_(ch), due to the increase in the retention of amylopectin-type fraction in the AF4 channel. Increased retention of the amylopectin-type fraction in the AF4 channel was also observed at low F ch and high F cr/F ch ratios. Large amylopectin-type molecules/particles (possibly aggregates) eluted at high F_(ch), low F_(cr) and low F_(cr)/F_(ch) ratios.