Hydrodynamic chromatography for the size classification of micron andsub-micron sized packing materials

摘要

Hydrodynamic chromatography (HDC) was used as a size classification and purification method forporous bridged ethyl hybrid (BEH) packing materials (particles) in the micron to sub-micron range.Using packed column HDC, a batch of particles with size 0.76 ± 0.26 μm was fractionated to yield clas-sified material of 1.05 ± 0.16 pm, reducing the relative standard deviation from 33% to 15%. Subsequentchromatographic evaluation of this packing material showed significant improvement in column per-formance and decrease in flow resistance over the unclassified material. Comparing a column packedwith the classified versus non-classified material, the effective flow resistance of the two columns wasdecreased by 58% and the minimum HETP for the packing material was improved from 4 to 2.5 p.m.