IN VITRO ANDROGENESIS OF BRASSICA OLERACEA VAR. CAPITATA L.

摘要

Anther culture technique provides an efficient method of creating homozygous lines in a very short period in comparison to conventional breeding methods. For practical breeding, it is important that, in anther culture, haploids can be recovered in high frequency from every donor plant. The tissue culture program to produce haploids in cabbage was started at the Institute in 1991. Aim of our experiments was to examine factors that might influence embryo production from anther culture of cabbage. Manyfactors may affect the yield of embryos, but the most important are the genotype, the conditions under wich the donor plants are raised, the size of the buds, the temperature and duration of the thermal shock, the medium composition.