An efficient plant regeneration protocol from seedling explants of Brassica juncea RH-781, a freeze tolerant cultivar

摘要

Brassica juncea is a major oilseed crop in India. There is a heavy loss of yield because of frost during flowering season. Two of the possible strategies considered to overcome this are transfer of genes responsible for frost tolerance and selectionof variant cells or in vitro raised plants using low temperature as selection pressure. Both of these require efficient regeneration protocols specific for each genotype. Here we present shoot regeneration protocol direct from seedling explants, which isbeing used in this laboratory.