盐和干旱胁迫对芥菜型油菜种子萌发及幼苗生长的影响

摘要

[目的]研究三种油菜种子萌发和幼苗生长对盐胁迫和干旱胁迫的响应,对不同种盐、旱胁迫油菜的耐受性提供科学参考.[方法]以三种芥菜型油菜为材料,通过不同浓度的NaCl和甘露醇处理,研究其对三种芥菜型油菜种子萌发和幼苗生长的影响.[结果](1)在盐和旱胁迫中,随着NaCl和甘露醇浓度的升高,其种子发芽率、根长和株高均呈下降趋势.NaCl(＜150 mmol/L)和甘露醇(＜200 mmol/L)时,三种芥菜型油菜种子发芽率跟对照组没有显著差异;NaCl(≥200 mmol/L)和甘露醇(≥300 mmol/L)时,显著抑制了其种子萌发,CBJ004-34油菜保持了78％的发芽率.(2)NaCl(＜150mmol/L)和甘露醇(＜100 mmol/L)时,Xinyou9和CBJ004-34油菜幼苗的根长跟对照没有显著差异;NaCl(＜100 mmol/L)时,促进新油9号油菜幼苗根的伸长.[结论]新油9号和CBJ004-34油菜种子的耐盐性和耐旱性好,该研究为选育油菜耐盐耐旱材料提供了参考依据.%[Objective] This project aims to carry out an evaluation of responses of seed germination and seedling growth of three cultivars of Brassica juncea to salt stress and drought stress in order to provide scientific reference for the tolerance of rape to different salt and drought stress.[Method]Three different cultivars of Brassica juncea were treated with different concentrations of NaCl and Mannitol to study the effects on seed germination and seedling growth of three kinds of Brassica juncea.[Result] (1) With the increase of NaCl and Mannitol concentration, the seed germination rate, plant height and seedling root length of three cultivars decreased. SG of the three cultivars at lower concentrations of NaCl (＜150 mmol/L) and Mannital (＜200 mmol/L) was similar to those of the control (SG 0 mmol/L); SG at higher concentrations of NaCl (≥200 mmol/L) and Mannital (≥300 mmol/L) significantly decreased, but SG of CBJ004-34 still exceeded 78％. (2) At lower concentrations of NaCl (＜150 mmol/L) and Mannital (＜100 mmol/L), the seedling root length of Xinyou 9 and CBJ004-34 were similar to that of the control. At lower concentration of NaCl (＜100 mmol/L), the seedling root length of Xinyou 9 was improved.[Conclusion]These results showed that Xinyou 9 and CBJ004-34 have good salt tolerance and drought tolerance, which has provided a reference for breeding rape salt and drought tolerant materials.