Water-Compatible Imprinted Polymers Based on CS@SiO_2 Particles for Selective Recognition of Naringin

摘要

Molecularly imprinted polymers are being proposed for the development of novel biorecognition elements for active components. In this study, an imprinted chitosan coated silica nanoparticles (I-CS@SiO_2) polymer was prepared by a simple procedure, in which, naringin (NG) with antioxidant activity, acted as a template, silica as a matrix and CS as a functional polymer. The binding properties were discussed by the equilibrium binding experiment method. Experiments show that the adsorption characteristics of I-CS@SiO_2 are better than that of nonimprinted polymer. It exhibited high selectivity for NG when compared with the nonimprinted polymer, with an imprinting factor a of 1.74. Scatchard analysis of the I-CS@SiO_2 indicated that there was a class of binding sites during the I-CS@SiO_2 recognizing NG: The dissociation constant of K_D is 0.016 mmol L~(-1), the maximum apparent binding capacity of B_(max) is 6.56 μmol g~(-1).