Competitive CE-UV Binding Tests For Selective Recognition Of Bisphenol A By Molecularly Imprinted Polymer Particles.

摘要

Capillary electrophoresis with ultraviolet detection (CE-UV) was used to perform competitive binding tests to demonstrate the selective recognition of bisphenol A (BPA) by molecularly imprinted polymer (MIP) particles. Cross-linking polymerization of methacrylic acid (MAA) and ethylene glycol dimethacrylate (EGDMA) in the presence of BPA yielded MIP particles with an average diameter of 164+/-15 nm. Their ability to recognize BPA in the presence of non-ionic, anionic and cationic water contaminants was investigated. Binding efficiency was rapidly determined, after sequential injection of particles first and compounds next into the fused silica capillary provided a short overlapping time during their electrophoretic migrations. The MIP particles exhibited high binding efficiency (99+/-1%) for BPA, which was not kinetically influenced by the applied voltage. Neither diclofenac nor metformin affected BPA binding, and 2-hydroxy4-methoxybenzophenone was even displaced from the particles by BPA. These results verified the high selectivity of MIP towards its target compound. Rapid CE-UV desorption test was successfully demonstrated using 5 % TEA in methanol. Reusability of MIP particles over several adsorption cycles without regeneration of the particles by desorbing the already bound analytes was confirmed as MIP particles can still remove a fairly high percent of BPA in the next two injections (2nd and 3rd) even in the presence of large quantities of HMB.