Purification and Characterization of the 7S Vicilin from Korean Pine(Pinus koraiensis)

摘要

Pine nuts are economically important as a source of human food.They are also of medical importance because numerous pine nut allergy cases have been recently reported.However,little is known about the proteins in pine nuts.The purpose of this study was to purify and characterize pine nut storage proteins.Reported here is the first detailed purification protocol of the 7S vicilin-type globulin from Korean pine(Pinus koraiensis)by gel filtration,anion exchange,and hydrophobic interaction chromatography.Reducing SDS-PAGE analysis indicated that purified vicilin consists of four major bands,reminiscent of post-translational protease cleavage of storage proteins during protein body packing in other species.The N-terminal ends of vicilin peptides were sequenced by Edman degradation.Circular dichroism(CD)and differential scanning calorimetry(DSC)analyses revealed that pine nut vicilin is stable up to 80°C and its folding-unfolding equilibrium monitored by intrinsic fluorescence can be interpreted in terms of a two-state model.