Separation and Identification of Phenolic Compounds in Olive Oil by Coupling High-Performance Liquid Chromatography with Postcolumn Solid-Phase Extraction to Nuclear Magnetic Resonance Spectroscopy(LC-SPE-NMR)

摘要

This study reports the first application of the hyphenated LC-SPE-NMR technique using postcolumn solid-phase extraction to the direct analysis of phenolic compounds in the polar part of olive oil.Apart from the identification and structure elucidation of simple phenols(hydroxytyrosol,tyrosol,vanillic acid,vanillin,p-coumaric acid,hydroxytyrosol,and tyrosol acetates),lignans(pinoresinol and 1-acetoxypinoresinol),flavonoids(apigenin and luteolin),and a large number of secoiridoid derivatives,this technique enables the identification of several new phenolic components,which had not been reported previously as constituents in the polar part of olive oil.