Immune tolerance of amniotic fluid stem cell-induced rat kidney graft and influences on oxidative stress

摘要

Objective This study aimed to use amniotic fluid stem cells of donors to induce immune tolerance of heterogenous rat kidney graft for investigating the formation mechanism of immune tolerance. Methods With Wistar rats as donors and Sprague-Dawley (SD) rats as receptors, the heterogenous kidney graft animal model was established, and amniotic fluid stem cells of Wistar rats were isolated and cultured. Moreover, 40 SD rats were randomly divided into 4 groups. Creatinine (Cr), blood urea nitrogen (BUN), interleukin (IL) 2, interferon (IFN) γ, and oxidative stress levels in serum were detected, flow cytometry was used to detect changes of CD4 and CD8 cells, and quantitative changes of urinary protein and pathologic changes of transplanted kidney were observed. Results BUN, Cr, IL-2, IFN-γ, and oxidative stress levels and urinary protein quantity in rat serum of the test group were significantly lower than those of the control group, creatinine clearance rate was significantly higher than that of the control group, and renal pathologic injury extent was significantly milder than that of the control group. Conclusions Amniotic fluid stem cells can induce immune tolerance of rat kidney graft and inhibit oxidative stress level, improve kidney function, and alleviate kidney injury.