Pyrazolo[1,5?a]?1,3,5-triazine C?Nucleoside as Deoxyadenosine Analogue: Synthesis, Pairing, and Resistance to Hydrolysis

摘要

The synthesis of a pyrazolo[1,5-a]-1,3,5-triazine C-nucleoside (dA~(PT)), designed to form two hydrogen bonds with a complementary dT residue, is reported. Oligonucleotides including this dA nucleoside analogue possess basepairing properties similar to those of the parent oligonucleotide. This dA nucleoside analogue is more resistant to acidcatalyzed hydrolysis than dA.