Quantitative Detection of Leglonella pneumophila in Water Samples: Assay Transfer to the LightCycler 480 Real-Time PCR System

摘要

An assay to detect and quantify Leglonella spp. in water samples was successfully transferred from a capillary-based LightCycler Instrument to the novel multiwell plate-based LightCycler 480 System. A previously described protocol was easily adapted by modifying the fluorescent label of the detection probe to match the filter set of the LightCycler 480 Instrument. While reproducibility and analytical sensitivity of the assay were found to be comparable on both systems, the novel plate-based instrument allowed for higher sample throughput and reduced assay time per sample.