首页> 外文期刊>Spectrochimica acta, Part A. Molecular and biomolecular spectroscopy >Binding interaction of differently charged fluorescent probes with egg yolk phosphatidylcholine and the effect of beta-cyclodextrin on the lipid-probe complexes: A fluorometric investigation
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Binding interaction of differently charged fluorescent probes with egg yolk phosphatidylcholine and the effect of beta-cyclodextrin on the lipid-probe complexes: A fluorometric investigation

机译:不同电荷的荧光探针与蛋黄磷脂酰胆碱的结合相互作用以及β-环糊精对脂质-探针复合物的影响:荧光检测

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摘要

Interaction of cationic phenosafranin (PSF), anionic 8-anilino-1-naphthalene sulfonate (ANS) and nonionic nile red (NR) have been studied with the zwitterionic phospholipid, egg yolk L-alpha-phosphatidylcholine (EYPC). The study reveals discernible binding interactions of the three fluorescent probes with the EYPC lipid vesicle. Once the binding of the probes with the lipid is established, the effect of cyclic oligosaccharide, beta-cyclodextrin (beta-CD), on these lipid bound probes has been investigated. Different fluorometric techniques suggest that addition of beta-CD to the probe-lipid complexes leads to the release of the probes from the lipid medium through the formation of probe-beta-CD inclusion complexes. A competitive binding of the probes between beta-cyclodextrin and the lipid is ascribed to be responsible for the effect. This provides an easy avenue for the removal of the probe molecules from the lipid environment. Extension of this work with drug molecules in cell membranes is expected to give rise to a strategy for the removal of adsorbed drugs from the cell membranes by the use of non-toxic beta-cyclodextrin. (C) 2015 Elsevier B.V. All rights reserved.
机译:已研究了阳离子苯酚弗拉宁(PSF),阴离子8-苯胺-1-萘磺酸盐(ANS)和非离子尼罗红(NR)与两性离子磷脂,蛋黄L-α-磷脂酰胆碱(EYPC)的相互作用。该研究揭示了三种荧光探针与EYPC脂质囊泡的可识别的结合相互作用。一旦建立了探针与脂质的结合,就研究了环状寡糖β-环糊精(β-CD)对这些脂质结合探针的作用。不同的荧光技术表明,将β-CD添加到探针-脂质复合物中会导致探针通过形成探针-β-CD包合物而从脂质介质中释放出来。 β-环糊精与脂质之间的探针竞争性结合是造成这种作用的原因。这为从脂质环境中除去探针分子提供了简便的途径。预期将这种工作扩展到细胞膜中的药物分子将产生一种通过使用无毒β-环糊精从细胞膜中去除吸附药物的策略。 (C)2015 Elsevier B.V.保留所有权利。

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