Optimization of Various Parameters for Maximum Production of Glucoamylaze by Neurospora sitophila

摘要

Glucoamylaze production by Neurospora sitophila was investigated under solid state fermentation. A number of process parameters including moisture content (%), pH, incubation temperature (°C), inoculum size (%), nutrients and duration of fermentation (h) were optimized using response surface methodology. The statistical results showed that the best culture conditions for glucoamylaze production were pH 5, temperature 37.5 °C and 72 h of incubation period while the optimum medium conditions were 15 g wheat bran, moisture content 65 %, inoculum level 20 %, starch 1.17 %, NH4NO3 0.66 % and SDS 0.3 % of the substrate weight. Under these optimized conditions, the enzyme production was enhanced to 335.2 ±12 U/gds which is approximately 2.6-fold increase in the enzyme production when compared with the initial fermentation conditions. To our best of knowledge, this is the first report on optimization of process variables using response surface methodology for glucoamylaze production under solid state fermentation from Neurospora sitophila.