Determination of Monoamine Neurotransmitters in Brains of Paraquat-Induced Mouse by HPLC with Electrochemical Detection

摘要

A liquid ehromatography-electrochemical detection method for the quantification of dopamine, 3,4-dihydroxyphenyl acetic acid, homovanillic acid, 3-methoxy-4-hydroxy phenylglycol, 5-hydroxytryptamine and 5-hydroxyindole-3-acetic acid was presented. The neurotransmitters were analyzed by an liquid chromatography on a C_(18) column with an electrochemical detection. Brain samples with 1 mL of 5 % sulfosalicylic acid were homogenized and centrifuged, then for analysis. This method exhibited excellent linearity for all of the analytes with regression coefficients higher than 0.99. The limit of detection values for dopamine, 3,4-dihydroxyphenyl acetic acid, homovanillic acid, 3-methoxy-4-hydroxy phenylglycol, 5-hydroxytryptamine and 5-hydroxyindole-3-acetic acid were 2.2, 3.0, 1.7, 3.7, 0.9 and 1.0 ng/mL, respectively. The precision results were expressed as relative standard deviation, ranging from 2.1 to 3.8 % for intra-day and from 2.8 to 4.8 % for the inter-day. The recoveries were ranged from 88.6 to 94.5 % for intra-day and from 86.6 to 93.9 % for the inter-day. This novel HPLC-ECD approach is precise, highly sensitive, specific and sufficiently simple.