Cellular Analysis and Detection Using Surface Plasmon Resonance Techniques

摘要

The spectrum of cell analysis applications described in this Review illustrates the growing level of interest and activity in this area. The combination of quantitative and label-free analysis with SPR provides interesting and new opportunities for fundamental and applied research. The examples above demonstrate the ability to manipulate and characterize cells on surfaces with SPR, and this technology will further our understanding of cellular behavior in physiologically relevant contexts. We expect that much more engineering and customization effort will go into SPR instrument development in the coming years to expand analysis capabilities and couple SPR with other analytical techniques for both mammalian and bacterial cells and that the use of SPR for cellular analysis will increase exponentially as the instruments continue to decrease in price and become readily available. Meanwhile, efforts to detect cells in complex samples using SPR are progressing rapidly, and the focus will soon shift from "proof-of-concept" biosensors to commercially available functionalized sensing chips and specialized SPR systems for agricultural, diagnostic, and other point-of-care applications.