Bioluminescent Probe for Hydrogen Peroxide Imaging in Vitro and in Vivo

摘要

Reactive oxygen species (ROS) often have significant roles in mediating redox modifications and other essential physiological processes, such as biological process regulation and signal transduction. Considering that H_2O_2 is a substantial member of ROS, detection and quantitation of H_2O_2 undertakes important but urgent responsibility. In this report, a bioluminescent probe for detecting H_2O_2 was well designed, synthesized, and evaluated. This probe was designed into three parts: a H_2O_2-sensitive aryl boronic acid, a bioluminescent aminoluciferin moiety, and a self-immolative linker. After extensive evaluation, this probe can selectively and sensitively react with H_2O_2 to release aminoluciferin. It should be pointed out that this probe is a potential bioluminescent sensor for H_2O_2 since it can provide a promising toolkit for real-time detection of the H_2O_2 level in vitro, in cellulo, and in vivo.