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Color Difference Amplification between Gold Nanoparticles in Colorimetric Analysis with Actively Controlled Multiband Illumination

机译:主动控制多波段照明的比色分析中金纳米粒子之间的色差放大

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Spectral chemical sensing with digital color analysis by using consumer imaging devices could potentially revolutionize personalized healthcare. However, samples with small spectral variations often cannot be differentiated in color due to the nonlinearity of color appearance. In this study, we address this problem by exploiting the color image formation mechanism in digital photography. A close examination of the color image processing pipeline emphasizes that although the color can be represented digitally, it is still a reproducible subjective perception rather than a measurable physical property. That makes it possible to physically manage the color appearance of a nonradiative specimen through engineered illumination. By using scattering light imaging of gold nanoparticles (GNPs) as a model system, we demonstrated via simulation that enlarged color difference between spectrally close samples could be achieved with actively controlled illumination of multiple narrow-band light sources. Experimentally, darkfield imaging results indicate that color separation of single GNPs with various sizes can be significantly improved and the detection limit of GNP aggregation-based colorimetric assays can be much reduced when the conventional spectrally continuous white light was replaced with three independently intensity-controlled laser beams, even though the laser lines were uncorrelated with the LSPR maxima of the GNPs. With low-cost narrow-band light sources widely available today, this actively controlled illumination strategy could be utilized to replace the spectrometer in many spectral sensing applications.
机译:通过使用消费类成像设备进行数字颜色分析的光谱化学传感可能会彻底改变个性化医疗保健。但是,由于颜色外观的非线性,具有较小光谱变化的样品通常无法区分颜色。在这项研究中,我们通过利用数字摄影中的彩色图像形成机制来解决这个问题。对彩色图像处理管道的仔细检查强调,尽管可以用数字表示颜色,但是颜色仍然是可复制的主观感觉,而不是可测量的物理属性。这使得可以通过工程照明物理地管理非辐射样本的颜色外观。通过使用金纳米颗粒(GNP)的散射光成像作为模型系统,我们通过仿真证明,可以通过主动控制多个窄带光源的照明来实现光谱接近的样本之间的色差增大。实验上,暗场成像结果表明,用三个独立的强度控制激光代替常规光谱连续白光,可以显着改善各种大小的单个GNP的颜色分离,并且可以大大降低基于GNP聚集的比色测定的检测极限。即使激光线与GNP的LSPR最大值无关,也不会产生光束。随着当今低成本低成本窄带光源的广泛普及,这种主动控制的照明策略可用于取代许多光谱传感应用中的光谱仪。

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