cat-ELCCA: A Robust Method To Monitor the Fatty Acid Acyltransferase Activity of Ghrelin O-Acyltransferase (GOAT)

摘要

Assays armed with catalytic signal amplification have arisen as superior systems for ultrasensitive detection of analytes. In the biocatalysis field, while fluorogenic and colorimetric enzyme assays designed to examine cleavage reactions have taken advantage of this strategy, many important enzyme classes, including acyltransferases, have mechanistically been excluded from such analytical techniques.