Glucocorticoid acts on a putative g protein-coupled receptor to rapidly regulate the activity of NMDA receptors in hippocampal neurons

摘要

Glucocorticoids (GCs) have been demonstrated to act through both genomic and nongenomic mechanisms. The present study demonstrated that corticosterone rapidly suppressed the activity of N-methyl-D-aspartate (NMDA) receptors in cultured hippocampal neurons. The effect was maintained with corticosterone conjugated to bovine serum albumin and blocked by inhibition of G protein activity with intracellular GDP-(??-S application. Corticosterone increased GTP-bound G s protein and cyclic AMP (cAMP) production, activated phospholipase C(?? 3 (PLC-P??), and induced inositol-1,4,5-triphosphate (IP 3) production. Blocking PLC and the downstream cascades with PLC inhibitor, IP3 receptor antagonist, Ca 2+ chelator, and protein kinase C (PKC) inhibitors prevented the actions of corticosterone. Blocking adenylate cyclase (AC) and protein kinase A (PKA) caused a decrease in NMDA-evoked currents. Application of corticosterone partly reversed the inhibition of NMDA currents caused by blockage of AC and PKA. Intracerebroventricular administration of corticosterone significantly suppressed long-term potentiation (LTP) in the CA1 region of the hippocampus within 30 min in vivo, implicating the possibly physiological significance of rapid effects of GC on NMDA receptors. Taken together, our results indicate that GCs act on a putative G protein-coupled receptor to activate multiple signaling pathways in hippocampal neurons, and the rapid suppression of NMDA activity by GCs is dependent on PLC and downstream signaling. ? 2012 the American Physiological Society.