Inosine and equilibrative nucleoside transporter 2 contribute to hypoxic preconditioning in the murine cardiomyocyte HL-1 cell line

摘要

We previously used the murine cardiomyocyte HL-1 cell line to investigate the role of ENTs in hypoxic or pharmacological PC and shown that PC involves adenosine, mouse (m)ENTl, and adenosine receptor activation (5, 6). However, HL-1 cells also possess ENT2, which has been reported to transport purine nucleosides and to have a higher affinity for inosine, a major and metabolically stable adenosine metabolite (8, 31). A growing body of data suggests that other purine nucleosides or nucleobases (e.g., inosine and adenine) have physiologically relevant actions in other tissues as a result of cell surface receptor signaling (7, 29, 30, 33).