Angiotensin-(l-7) serves as an aquaretic by increasing water intake and diuresis in association with downregulation of aquaporin-1 during pregnancy in rats

摘要

Animals. Timed-pregnant and virgin female Sprague-Dawley rats between 12 and 14 wk of age were obtained from Harlan Laboratories (Indianapolis, IN) and housed individually under a 12:12-h light-dark cycle in a facility approved by the Association for Assessment and Accreditation of Laboratory Animal Care. Day 0 of pregnancy was designated as the day when sperm were found in the vaginal smear. All protocols were approved by the Animal Care and Use Committee of Wake Forest University School of Medicine and are in compliance with National Institutes of Health guidelines.