Biomedical Potential of Ultrafine Ag/AgCl Nanoparticles Coated on Graphene with Special Reference to Antimicrobial Performances and Burn Wound Healing

摘要

In recent years, researchers have proven the release of silver ions (Ag+) from silver nanoparticles (Ag NPs) significantly affects their toxicity to bacteria and other organisms. Due to the difficulty in maintaining a steady flux of a high concentration of Ag+, it is still challenging to develop a highly efficient, stable, and biocompatible Ag NP-based antimicrobial material. To circumvent this issue, we developed a new Ag-based bactericide through the fabrication of sunlight driven and ultrafine silver/silver chloride anchored on reduced graphene oxide (Ag/AgCl/rGO). This stable Ag/AgCl nanophotocatalyst with negligible release of Ag+ generated a high amount of oxidative radicals, killing the bacteria, thus achieving both high bactericidal efficiency and stability. Moreover, functionalization of the nanomaterial with poly(diallyldimethylammonium chloride) (PDDA) gives it a highly adsorptive capacity, which allows it to capture the bacteria and possibly enhances the bactericidal activity. In vivo histopathological studies showed that the Ag/AgCl/rGO nanomaterial could obviously promote the regeneration of the epidermis, which indicated the good biomedical potential of Ag/AgCl/rGO nanomaterial in burn wound healing.