Identification of an AFLP molecular marker linked to parthenocarpy gene in monoecious cucumber.

摘要

In order to identify molecular markers linked to parthenocarpy in cucumber (Cucumis sativus L.), parthenocarpy and non-parthenocarpy DNA pools were created from F₂ crosses between a highly parthenocarpic monoecious line '6457' and a non-parthenocarpic line '6429' according to the principle of bulk segregant analysis (BSA). The amplified fragment length polymorphism (AFLP) technique was employed with 256 primer combinations to detect the polymorphisms between the DNA pools. In total, 8544 scorable AFLP bands were generated, yielding an average of 33.38 fragments per primer combination. In the non-parthenocarpy DNA pool, a 325 bp specific fragment was amplified with the primer E41/M47, the band did not appear in the parthenocarpic pool. This marker, designated AGG/CAA₃₂₅ was validated with DNA from the F₂ population. Linkage analysis using MapMaker 3.0 indicated that the genetic distance from the marker to the non-parthenocarpy locus was 9.7 cM.