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首页> 外文期刊>Journal of Science and Technology of Agriculture and Natural Resources >Identification of AFLP Molecular Markers Linked to Row-Type Gene in Barley
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Identification of AFLP Molecular Markers Linked to Row-Type Gene in Barley

机译:大麦行型基因AFLP分子标记的鉴定

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Formation of the two- and six-rowed types in barley is predominantly controlled by alleles at a single locus (vrs1) which is located in long arm of chromosome 2H. This gene is a key character on the study of barley domestication and yield. Near-isogenic lines (NILs) of barley were produced from crosses between Kanto Nakate Gold (tow-rowed) and Azumamugi (six-rowed). The selected lines were used for screening of AFLP polymorphic bands which are linked to vrs1 locus. After screening of a total of 1792 primer combination, five polymorphic bands were identified. A construction of high resolution map around the vrs1 locus was made using recombinant inbred lines. These markers can be used for a map-based cloning of the genes at the vrs1 locus. Keywords: Barley, Row-type, Near-isogenic lines (NILs), aFLP, vrs1 Full-Text Type of Study: Research | Subject: Ggeneral Received: 2008/01/9 Related Websites Scientific Publications Commission - Health Ministry Scientific Publications Commission - Science Ministry Yektaweb Company Site Keywords ?????, Academic Journal, Scientific Article, ???? ????? ??, ???? ????? ??, ???? ????? ??, ???? ????? ??, ???? ????? ??, ???? ????? ??, ???? ?? Vote ? 2015 All Rights Reserved | JWSS - Isfahan University of Technology
机译:大麦中两行和六行类型的形成主要受位于2H染色体长臂的单个基因座(vrs1)的等位基因控制。该基因是大麦驯化和产量研究的关键特征。大麦的近等基因系(NILs)是由关东中田金(两行)和祖木(六行)之间的杂交产生的。选择的品系用于筛选与vrs1基因座相关的AFLP多态性条带。在筛选了总共1792个引物组合后,鉴定出五个多态性条带。使用重组自交系构建了围绕vrs1基因座的高分辨率图。这些标记可用于在vrs1基因座处基于图谱的基因克隆。关键字:大麦,行型,近等基因系(NIL),aFLP,vrs1主题:一般收稿日期:2008/01/9相关网站科学出版物委员会-卫生部科学出版物委员会-科学部Yektaweb公司网站关键字??????,Academic Journal,Scientific Article,???? ?????? ??,???? ?????? ??,???? ?????? ??,???? ?????? ??,???? ?????? ??,???? ?????? ??,???? ??投票吗? 2015版权所有| JWSS-伊斯法罕工业大学

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