Preliminary genomic and functional analysis of a cDNA library from Botrytis cinerea.

摘要

The disease "gray mold" is produced by the fungus Botrytis cinerea and is responsible for great economic losses on many crops such as berries, kiwifruits, tomatoes, apples, grapes and ornamentals. A better understanding of the physiology of B. cinerea physiology can be obtained by means of genomic tools. A cDNA library was built to allow the identification of fungal genes participating in infection, pathogenicity and tolerance to pesticides. The present work describes the construction, sequencing and bioinformatic analysis of a cDNA library prepared from an isolate of Botrytis cinerea obtained from blueberries. We are interested in isolating genes related to pathogenicity in soft berries, in particular raspberries. More than 11 000 EST were sequenced, assembled and annotated by comparing them with the available data bases. The results of the bioinformatics analysis indicated that more than 50% of the sequences identified are involved in cellular metabolism, 12% are involved in transport and approximately 10% in cellular organization. Other functional categories include genes involved in response to biotic and abiotic stimuli, cell communication, cell homeostasis and development. The analysis of this library has generated new information regarding the nature of the genes involved in spore attachment, spore germination, tissue penetration and tissue invasion.