Genetic diversity and differentiation among native populations of Lilium formosanum and L. Longiflorum of Taiwan based on molecular marker analysis.

摘要

To study the genetic diversity of Lilium formosanum and L. longiflorum native to Taiwan, germplasm from 32 native Lilium populations were collected from two distinct geographic districts: 'Central Mountain District' (northern, central and southern area, 3 areas and 19 population sites) and 'Coast District' (northeast, east and off shore islets, 3 areas and 13 population sites). This information can be used as a foundation for germplasm conservation and breeding. The genetic diversity and variance of L. formosanum and L. longiflorum native to Taiwan were analyzed using molecular markers with 8 pairs of microsatellite primers and 4 arbitrary decdamer oligonucleotides to carry on PCR reactions. Eighty six fragments were obtained from 480 plants representing 32 populations with an average of 7.2 fragments per primer pair. The IGD (Index of Genetic Distance) between germplasm groups were calculated using Nei's method. Shien-Wuan-Yiang and Wu-She (IGD=0.0184) were closely related, and Lan-Yu and Tuen-Yuan (IGD=0.1746) were most distantly related. UPGMA results showed that with the exception of Guan-Wu, populations group according to their geographic areas and can be divided into 'Central Mountain District' populations (L. formosanum) and 'Coast District' populations (L. longiflorum).