An approach to compare Affymetrix and cDNA gene expressions

摘要

The development of microarray technology for high-throughput measurement of gene expressions is proving a powerful means for studying the transcriptome on a genomic scale and across diverse biological systems and experimental designs. The technology has grown rapidly in academia, medicine and the pharmaceutical, biotechnology, agrochemical and food industries. The technology confers the freedom to conduct experiments in its multiple platforms and attends to the extensive molecular surveillance of cells and tissues. Despite a large number of studies available in the literature comparing results between the existing platforms remains a challenging task in microarray technology. In this direction, we have developed an approach that allows integration of microarray experiments between two of its platforms, i.e. Affymetrix (R) and cDNA microarrays. In this communication, we elaborate on our approach and present our validation. In addition, we have also examined our approach to evaluate where it stands amid a few existing methods.