首页> 外文期刊>Bioresource Technology: Biomass, Bioenergy, Biowastes, Conversion Technologies, Biotransformations, Production Technologies >Biosynthesis and characterization of polyhydroxyalkanoate containing high 3-hydroxyhexanoate monomer fraction from crude palm kernel oil by recombinant Cupriavidus necator
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Biosynthesis and characterization of polyhydroxyalkanoate containing high 3-hydroxyhexanoate monomer fraction from crude palm kernel oil by recombinant Cupriavidus necator

机译:重组铜核菌从粗棕榈仁油中生物合成和表征含3-羟基己酸高单体含量的聚羟基链烷酸酯

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摘要

The potential of plant oils as sole carbon sources for production of P(3HB-co-3HHx) copolymer containing a high 3HHx monomer fraction using the recombinant Cupriavidus necator strain Re2160/pCB113 has been investigated. Various types and concentrations of plant oils were evaluated for efficient conversion of P(3HB-co-3HHx) copolymer. Crude palm kernel oil (CPKO) at a concentration of 2.5g/L was found to be most suitable for production of copolymer with a 3HHx content of approximately 70mol%. The time profile of these cells was also examined in order to study the trend of 3HHx monomer incorporation, PHA production and PHA synthase activity. ~1H NMR and ~(13)C NMR analyses confirmed the presence of P(3HB-co-3HHx) copolymer containing a high 3HHx monomer fraction, in which monomers were not randomly distributed. The results of various characterization analyses revealed that the copolymers containing a high 3HHx monomer fraction demonstrated soft and flexible mechanical properties.
机译:研究了植物油作为唯一的碳源的潜在潜力,利用重组铜杯霉菌(Cupriavidus)硝化菌株Re2160 / pCB113生产含有高3HHx单体馏分的P(3HB-co-3HHx)共聚物。评价了各种类型和浓度的植物油,以有效转化P(3HB-co-3HHx)共聚物。发现浓度为2.5g / L的粗棕榈仁油(CPKO)最适合生产3HHx含量约为70mol%的共聚物。为了研究3HHx单体掺入,PHA产生和PHA合酶活性的趋势,还检查了这些细胞的时间分布。 〜1H NMR和〜(13)C NMR分析证实存在包含高3HHx单体分数的P(3HB-co-3HHx)共聚物,其中单体不是随机分布的。各种表征分析的结果表明,含有高3HHx单体分数的共聚物显示出柔软而灵活的机械性能。

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